Baccoside A suppresses epileptic-like seizure/convulsion in Caenorhabditis elegans

Abstract 

The 1mm long Caenorhabditis elegans is one of the prime research tools to study different human neurodegenerative diseases. We have considered the case in which increase in the surrounding temperature of this multicellular model leads to abnormal bursts of neuronal cells that can be linked to seizure or convulsion. The induction of such seizure/convulsion mechanism was done by gradually increasing the temperature with 1× buffer (100mM NaCl, 50mM MgCl₂) in adult C. elegans. In the present experiment it is demonstrated that Baccoside A can significantly reduce the seizure/convulsion in C. elegans at higher temperatures (26–28±1°C). Furthermore, in T-type Ca²⁺ channel cca-1 mutant worms, no convulsion was recorded. Our experimental results suggest that plant molecules from Bacopa monnieri may be useful in suppressing the seizure/convulsion in worms.

Keywords: Epileptic-like seizure, Convulsion, Ca²⁺ oscillations, T-type calcium channel, Baccoside A, Caenorhabditis elegans