Seizure: European Journal of Epilepsy
Volume 19, Issue 4 , Pages 205-210, May 2010

Byproducts of protein, lipid and DNA oxidative damage and antioxidant enzyme activities in seizure

  • Marko Ercegovac

      Affiliations

    • Institute of Neurology, Clinical Centre of Serbia, Dr Subotica 2, 11000 Belgrade, Serbia
    • ,
  • Nebojsa Jovic

      Affiliations

    • Clinic of Neurology and Psychiatry for Children and Youth, Dr Subotica 6a, 11000 Belgrade, Serbia
    • ,
  • Tatjana Simic

      Affiliations

    • Institute of Medical and Clinical Biochemistry, Medical Faculty, University in Belgrade, Pasterova 2, 11000 Belgrade, Serbia
    • ,
  • Ljiljana Beslac-Bumbasirevic

      Affiliations

    • Institute of Neurology, Clinical Centre of Serbia, Dr Subotica 2, 11000 Belgrade, Serbia
    • ,
  • Dragoslav Sokic

      Affiliations

    • Institute of Neurology, Clinical Centre of Serbia, Dr Subotica 2, 11000 Belgrade, Serbia
    • ,
  • Tatjana Djukic

      Affiliations

    • Institute of Medical and Clinical Biochemistry, Medical Faculty, University in Belgrade, Pasterova 2, 11000 Belgrade, Serbia
    • ,
  • Ana Savic-Radojevic

      Affiliations

    • Institute of Medical and Clinical Biochemistry, Medical Faculty, University in Belgrade, Pasterova 2, 11000 Belgrade, Serbia
    • ,
  • Marija Matic

      Affiliations

    • Institute of Medical and Clinical Biochemistry, Medical Faculty, University in Belgrade, Pasterova 2, 11000 Belgrade, Serbia
    • ,
  • Jasmina Mimic-Oka

      Affiliations

    • Institute of Medical and Clinical Biochemistry, Medical Faculty, University in Belgrade, Pasterova 2, 11000 Belgrade, Serbia
    • ,
  • Marija Pljesa-Ercegovac

      Affiliations

    • Institute of Medical and Clinical Biochemistry, Medical Faculty, University in Belgrade, Pasterova 2, 11000 Belgrade, Serbia
    • Corresponding Author InformationCorresponding author. Tel.: +381 11 3643249; fax: +381 11 3643270.

Received 5 October 2009; received in revised form 25 January 2010; accepted 5 February 2010. published online 12 March 2010.

Abstract 

Purpose

To get more insight into molecular mechanisms underlying oxidative stress and its role in different types of seizure, in this study, oxidative byproducts of proteins, lipids and DNA, as well as, antioxidant enzyme activities were studied in adult patients with epilepsy.

Methods

Study was performed in 60 patients with epilepsy and in 25 healthy controls. Plasma protein reactive carbonyl derivatives (RCD) and protein thiol groups (P-SH), byproducts of oxidative protein damage, as well as antioxidant enzyme activities, superoxide dismutase (SOD) and glutathione peroxidase (GPX) were studied spectrophotometrically. Urinary 8-epi-prostaglandin F2α (8-epi-PGF2α) and 8-hydroxy-2′-deoxyguanosine (8-OHdG), representative byproducts of lipid and DNA oxidative damage, respectively, were determined by enzyme immunoassay.

Results

RCD levels were significantly increased (p=0.001), while P-SH content was decreased in patients with first seizure (p=0.052) compared to controls, independently of the seizure type. Urinary 8-epi-PGF2α and 8-OHdG were significantly increased in patients with epilepsy (p=0.001 and p=0.001). Rise in 8-epi-PGF2α was more pronounced in patients with generalized tonic–clonic seizure (GTCS) compared to those with partial seizure (PS). Both SOD and GPX activity were significantly increased in epileptic patients compared to controls (p=0.001 and p=0.001), but only SOD activity was significantly higher in patients with GTCS than in those with PS.

Conclusions

Data on enhanced protein, lipid and DNA oxidation, together with upregulated antioxidant enzyme activities, confirm the existence of systemic oxidative stress in patients with epilepsy. It might be speculated that post-translational modification to existing functional proteins, particularly alterations to ion channels, might be at least partially responsible for acute early changes in neuronal networks.

Keywords: Epilepsy, Seizure, Oxidative stress, Antioxidant activity, Carbonyl groups, Urinary isoprostanes

 

PII: S1059-1311(10)00031-2

doi:10.1016/j.seizure.2010.02.002

Seizure: European Journal of Epilepsy
Volume 19, Issue 4 , Pages 205-210, May 2010

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