Seizure: European Journal of Epilepsy
Volume 19, Issue 3 , Pages 165-172 , April 2010

Oxidative stress mediates hippocampal neuron death in rats after lithium–pilocarpine-induced status epilepticus

  • Jinzhi Liu

      Affiliations

    • Department of Neurology, Qianfoshan Hospital, Medical School of Shandong University, No. 66, Jingshi Road, Jinan, Shandong 250014, PR China
    • ,
  • Aihua Wang

      Affiliations

    • Department of Neurology, Qianfoshan Hospital, Medical School of Shandong University, No. 66, Jingshi Road, Jinan, Shandong 250014, PR China
    • Corresponding Author InformationCorresponding author. Tel.: +86 13791120819.
    • ,
  • Lili Li

      Affiliations

    • Department of Histology and Embryology, Shandong University, Jinan, Shandong 250012, PR China
    • ,
  • Yanfei Huang

      Affiliations

    • Department of Neurology, Qianfoshan Hospital, Medical School of Shandong University, No. 66, Jingshi Road, Jinan, Shandong 250014, PR China
    • ,
  • Ping Xue

      Affiliations

    • Department of Neurology, Qianfoshan Hospital, Medical School of Shandong University, No. 66, Jingshi Road, Jinan, Shandong 250014, PR China
    • ,
  • Aijun Hao

      Affiliations

    • Department of Histology and Embryology, Shandong University, Jinan, Shandong 250012, PR China

Received 5 October 2009 ,Revised 12 January 2010 ,Accepted 15 January 2010.

  • Image Result

    High power (40×) photomicrographs showing H&E staining with hematoxylin and eosin of the hippocampal CA3 pyramidal neurons at 6;h and 7 days rats after LPCSE. (A) The changes of the hippocampal CA3 pyr

    High power (40×) photomicrographs showing H&E staining with hematoxylin and eosin of the hippocampal CA3 pyramidal neurons at 6h and 7 days rats after LPCSE. (A) The changes of the hippocampal CA3 pyramidal neurons at 6h and 7 days in rats after SE with or without FK506. Bar=20μm. (A′) The number of surviving cells after SE. Compared with control rats, the surviving cells of rats of SE group showed decrease significantly. FK506 could keep from cells damage. Bars indicate mean±SD. *P<0.05 vs. control. #P<0.05 vs. pilo

  • Image Result
    Changes of NO level, cNOS and iNOS activity after LPCSE. (A) Changes of NO level after SE; (A′ and A″) the effects of FK506 on NO level at 6h and 7 days after SE. (B) Changes of cNOS activity after SE

    Changes of NO level, cNOS and iNOS activity after LPCSE. (A) Changes of NO level after SE; (A′ and A″) the effects of FK506 on NO level at 6h and 7 days after SE. (B) Changes of cNOS activity after SE; (B′) the effects of FK506 on cNOS activity at 6h after SE. (C) Changes of iNOS activity after SE; (C′) the effects of FK506 on iNOS activity at 7 days after SE. Bars indicate mean±SD. *P<0.05, **P<0.01 vs. control. #P<0.05 vs. pilo and or saline+pilo.

  • Image Result
    Changes of MDA level and SOD activities after SE induced by LPCSE. (A) Changes of MDA level after SE; (A′) the effects of FK506 on MDA level at 7 days after SE. (B) Changes of SOD activity after SE; (

    Changes of MDA level and SOD activities after SE induced by LPCSE. (A) Changes of MDA level after SE; (A′) the effects of FK506 on MDA level at 7 days after SE. (B) Changes of SOD activity after SE; (B′ and B″) the effects of FK506 on SOD activity at 2h and 7 days after SE. Bars indicate mean±SD. *P<0.05, **P<0.01 vs. control. #P<0.05 vs. pilo or saline+pilo.

  • Image Result
    The expression positive cells of nNOS, iNOS and caspase-3. (A, A′ and A″, 400×) Immunohistochemistry showed the effects of FK506 on positive cells of nNOS at 6h after SE. (B, B′ and B″, 600×) Immunohi

    The expression positive cells of nNOS, iNOS and caspase-3. (A, A′ and A″, 400×) Immunohistochemistry showed the effects of FK506 on positive cells of nNOS at 6h after SE. (B, B′ and B″, 600×) Immunohistochemistry showed the effects of FK506 on positive cells of iNOS at 7 days after SE. (C, C′ and C″, 600×) Immunohistochemistry showed the effects of FK506 on positive cells of caspase-3 at 7 days after SE. Bars indicate averages±SD. *P<0.05, **P<0.01 vs. control. #P<0.05 vs. pilo or saline+pilo.

  • Image Result
    Protein expression of nNOS, iNOS and caspase-3 after SE. (A and A′) The changes of protein expression of nNOS, iNOS and caspase-3 after SE in different times. (B and B′) Protein expression of nNOS and

    Protein expression of nNOS, iNOS and caspase-3 after SE. (A and A′) The changes of protein expression of nNOS, iNOS and caspase-3 after SE in different times. (B and B′) Protein expression of nNOS and the effect of FK506 on nNOS at 6h after SE. (C and C′) Protein expression of iNOS and the effect of FK506 on iNOS at 7 days after SE. (D and D′) Protein expression of caspase-3 and the effect of FK506 on caspase-3 at 7 days after SE. Bars indicate averages±SD. *P<0.05, **P<0.01 vs. control. #P<0.05 vs. pilo or saline+pilo.

PII: S1059-1311(10)00011-7

doi: 10.1016/j.seizure.2010.01.010

Seizure: European Journal of Epilepsy
Volume 19, Issue 3 , Pages 165-172 , April 2010

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